Table 1 Primers used in this study
Gene | Primer | Sequence (5'-3') | Size |
|---|---|---|---|
Actin (S. scabiei) | SsAct F | CAACCATCCTTCTTGGGTATG | 311 bp |
SsAct R | CCAGCTTCGTCGTATTCTTGT | ||
Actin (P. ovis) | PoAct F | CATCAAGGTGTCATGGTTGG | 225 bp |
PoAct R | GGCTTTTGGATTCAATGGAG | ||
Actin (P. pastoris) | PpAct F | AGCGCGCCCATTTCTTACCGT | 203 bp |
PpAct R | TCTCCGGCGTATCCGGCCTT | ||
EF1a (S. scabiei) | SsEF1a F | TTGGCTTATACCTTGGGTGTG | 181 bp |
SsEF1a R | CACCGTTCCATCCAGAGATT | ||
EF1a (D. pteronyssius) | DpEF1a F | CCCGTGAACATGCTTTGCTTGCC | 175 bp |
DpEF1a R | ACAAAAGCGACGGTAGCCGGA | ||
EF1a (P. ovis) | PoEF1a F | CAAATATGCCTGGGTTTTGG | 205 bp |
PoEF1a R | TTCACCAGTACCAGCAGCAA | ||
EF3 (P. pastoris) | PpEF3 F | GTCGCCATTTTGGATGCTAT | 251 bp |
PpEF3 R | AGGGTGTGGACAGTTTCTGG |