Immunoregulation of bovine macrophages by factors in the salivary glands of Rhipicephalus microplus

Parasites & Vectors

Table 1 Genes analysed by Taqman quantitative real-time PCR

Gene name	NCBI Accession	Sense	Anti-sense	Probe: FAM-TAMRA
CD86	NM_001038017	TAAGGCCGACAGCAGTTTCC	TCACCCCGTTATTAAGATGATAGC	TCCCAGCTCTGCTTCCAGTCGGGT
CD80	Y09950	GCATTGTGATCCTGGCTCTG	CTGATCATTAACCTCACGGAAGTC	TGTCGGACAGTGGCACCTACACCT
CD40	NM_001105611	GCATTGTGATCCTGGCTCTG	CTGATCATTAACCTCACGGAAGTC	TGTCGGACAGTGGCACCTACACCT
CD69	NM_174014	ACCTTGGCCCAAAACTTTTGC	CAGCCCGATCCAGTGTTCAG	AACATGGTGCCACGCTTGCTGTCA
IFN-γ	NM_174086	GAAAGCGGAAGAGAAGTCAGAATC	CAAATATTGCAGGCAGGAGGAC	ACGTTGATGCTCTCCGGCCTCGAA
IL-12p40	NM_174356	TGTGACACTCCTGAAGAAGATGG	CCAAACTCTTTGACTTGGATGGTC	TGCCAGAGCCCAAGACCTCACTGC
IL-10	NM_174088	CTCTGTTGCCTGGTCTTCCTG	TGGTTGGCAAGTGGATACAGC	CAGCCAGCCGAGATGCGAGCACC
IL-4	NM_173921	AGCAAGACCTGTTCTGTGAATG	CAGCTTCAACACTTGGAGTATTTC	AGCCAAGACGAGCACAAGTACGCT

Gene names, NCBI gene accession, sense and anti-sense primer sequences (5' to 3') and Taqman probe sequence.

ISSN: 1756-3305