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Figure 1 | Parasites & Vectors

Figure 1

From: Real-time PCR of the mammalian hydroxymethylbilane synthase (HMBS) gene for analysis of flea (Ctenocephalides felis) feeding patterns on dogs

Figure 1

Alignment of HMBS amplification targets of Canis lupus familiaris , Felis catus , Equus caballus , Bos taurus , and jewel wasp Nasonia vitripennis. Dots indicate nucleotides identical to the canine HMBS sequence, and dashes represent nucleotide deletions. Primers and probes are shown by boxes. The upstream primer is used as shown while the downstream primer and probes are applied as reverse complement (antisense) oligonucleotides. The probes were designed for discrimination of the homologous sequences of different animal species. K represents G or T, and R stands for A or G.

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