Occurrence and identification of risk areas of Ixodes ricinus-borne pathogens: a cost-effectiveness analysis in north-eastern Italy

Table 2 Primers and UPL used for genospecies identification of Borrelia burgdorferi s.l. in co-infected ticks using real time PCR assays

*Genospecies*	*Target gene*	*5'→ 3'primer sequence*	*UPL number*	Amplicon size (bp)^c
B. burgdorferi s.s.	OspA^a	TCTTGAAGGAACTTTAACTGCTGA TGAAACTTCCCCAGATTTTGA	#119	97
B. afzelii	OspA	GACTCCGCAGGTACCAATTT AAAGCGTTTTTAAGTTCATCAAGTG	#98	71
B. garinii	Fla^b	TCTGCTATGATTATGCCACCA CCTTTGCCTAAGAATTGATTACCA	#2	74
B. valaisiana	Fla	CCAAATGCACATGTTGTCAAA TTTGCAGGTTGCATTCCA	#132	78

ISSN: 1756-3305