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Table 2 Primers used for amplification and sequencing of gltA and GroEL genes of N. mikurensis , and the amplification of the Msp2 gene of A. phagocytophilum

From: Prevalence of Neoehrlichia mikurensis in ticks and rodents from North-west Europe

Gene

Name

Type

Sequence

Reference

gltA

NMik fo-gltA

Primer (forward)

5’-aagtgcatgctttgctacatt-‘3

This study

gltA

NMik re-gltA

Primer (reverse)

5’-tcatgatctgcatgtaaaataaat-‘3

This study

GroEL

NMikGroEL-F2a

Primer (forward)

5’-ccttgaaaatatagcaagatcaggtag-‘3

This study

GroEL

NMikGroEL-R2b

Primer (reverse)

5’-ccaccacgtaacttatttagtactaaag -‘3

This study

GroEL

NMikGroEL-P2a

Probe (RED)

5’-RED-cctctactaattattgctgaagatgtagaaggtgaagc-BHQ2-‘3

This study

GroEL

NMik fo-groEL

Primer (forward)

5’-gaagyatagtytagtatttttgtc-‘3

[18]

GroEL

NMik re-groEL

Primer (reverse)

5’-ttaacttctacttcacttgaacc-‘3

[18]

GroEL

NMik seq1groEL

Primer (reverse)

5’-acatcacgcttcatagaaag-‘3

[18]

GroEL

NMik seq2groEL

Primer (forward)

5’-aaaggaattagtattagaatcttt-‘3

[18]

GroEL

NMik seq3groEL

Primer (forward)

5’-aatatagcaagatcaggtagac-‘3

[18]

GroEL

NMik seq4groEL

Primer (reverse)

5’-cttccattttaactgctaattc-‘3

[18]

Msp2

ApMSP2F

Primer (forward)

5’-atggaaggtagtgttggttatggtatt-‘3

[26]

Msp2

ApMSP2R

Primer (reverse)

5’-ttggtcttgaagcgctcgta-‘3

[26]

Msp2

ApMSP2P

Probe (FAM)

5’-FAM-tggtgccagggttgagcttgagattg-BHQ1-‘3

[26]

  1. Primers were either identical to or slightly modified from the primers described in the reference papers.