Table 2 Experimental design of an ectoparasiticidal efficacy study against Rhipicephalus sanguineus and Ctenocephalides felis felis on dogs, Study 1
Study Day | Activity | ||
|---|---|---|---|
-6 | 24 dogs each infested with 100 fleas | ||
-5 | Fleas counted and flea numbers used for ranking and allocation to groups | ||
-1 | Allocation to control group of 8 dogs (Group 1) and two treated groups (Groups 2, 3) | ||
0 | Dogs infested with 100 fleas and 50 ticks immediately prior to collaring | ||
2 | 48 h flea and tick counts* | ||
7 | 50 ticks | ||
8 | 100 fleas | ||
9 | 24 h flea, 48 h tick counts | ||
14 to 30 | Infestation and counting at weekly intervals the same as for Days 7 to 9 above | ||
56 | 50 ticks | ||
57 | 100 fleas | ||
58 | 24 h flea, 48 h tick counts | ||
84 to 142 | Infestation and counting at 4-weekly intervals the same as for Days 56 to 58 above | ||
160 | Two fresh groups of 8 dogs each (Groups 4 and 5) added to the study | ||
161 | Infestation with fleas and ticks (Groups 1, 4 and 5), followed by spot-on treatment of dogs in groups 4 and 5. | ||
163 | Flea and tick counts 48 h after treatment on dogs in Groups 1, 4 and 5, thereafter dogs in all five groups subject to the same infestation and counting regimen | ||
168 | 50 ticks | ||
169 | 100 fleas | ||
170 | 24 h flea, 48 h tick counts | ||
170 | End of label claim for Group 3, no further evaluation | ||
175 to 198 | Infestation and counting at weekly intervals the same as for Days 168 to 170 above | ||
199 | End of label claim for Groups 4 and 5, no further evaluation | ||
224 | 50 ticks | ||
225 | 100 fleas | ||
226 | 24 h flea, 48 h tick counts | ||