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Table 3 Experimental design of an ectoparasiticidal efficacy study against Rhipicephalus sanguineus and Ctenocephalides felis felis on dogs, Study 2

From: Efficacy of slow-release collar formulations of imidacloprid/flumethrin and deltamethrin and of spot-on formulations of fipronil/(s) - methoprene, dinotefuran/pyriproxyfen/permethrin and (s) –methoprene/amitraz/fipronil against Rhipicephalus sanguineus and Ctenocephalides felis felis on dogs

Study day

Activity

-5 to -3

Flea infestation, counting and allocation to 3 groups of 8 dogs (Groups 1, 2, 3)

0

Controls (Group 1) and treated dogs (Groups 2 and 3) infested with fleas and ticks, and treated directly thereafter (Groups 2 and 3)

2

  

48 h flea + tick counts

7

50 ticks, 100 fleas

6 h tick counts

  

8

  

18 h tick counts

24 h flea counts

14 to 29

Infestation and counting at weekly intervals the same as for Days 7 and 8 above

34

Fresh group of 8 dogs (Groups 4) added to study

35

Groups 1, 2 and 3 infested with fleas + ticks, Group 3 dogs re-treated and Group 4 dogs treated for the first time directly thereafter

 

6 h tick counts (Groups 1, 2, and 3)

36

 

18 h tick counts and 24 h flea counts (Groups 1, 2, and 3)

42 (all groups)

50 ticks, 100 fleas

6 h tick counts

  

43

  

18 h tick counts

24 h flea counts

49 to 71

Infestation and counting at weekly intervals the same as for Days 42 and 43 above