Table 4 Effect of silymarin with/without praziquantel on hepatic hydroxyproline content and immunohistochemical expression of transforming growth factor-β1 and matrix metalloproteinase-2, 10 and 18 weeks post-infection of mice with S.mansoni
Animal groups | Within 10 successive microscopic fields (×400)/section/animal (Mean percentage +ve cells ± SEM) | Hydroxyproline content (μg/g liver) | ||||
|---|---|---|---|---|---|---|
TGF-β1 | MMP-2 | |||||
10 wks | 18 wks | 10 wks | 18 wks | 10 wks | 18 wks | |
Uninfected(vehicle) | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 | 789.45 ± 50.61 | 763.82 ± 22.32 |
Infected (Vehicle) | 50.60 ± 3.62 | 62.60 ± 4.21 | 37.00 ± 2.55 | 50.00 ± 5.70 | ††† 1495.88 ± 91.85 | ††† 1961.96 ± 80.97 |
Infected + PZQ | ‡‡‡ 14.80 ± 0.37 (70.75%) | ‡‡‡ 29.5 ± 2.01 (52.88%) | ‡ 26.0 ± 1.62 (29.72%) | ‡‡‡ 17.67 ± 1.82 (64.66%) | †‡‡‡ 983.57 ± 41.43 (34.25%) | †††‡‡‡ 1184.84 ± 77.27 (39.61%) |
Infected + silymarin | ‡‡‡ 25.50 ± 2.81 (49.60%) | ‡‡‡ 32.17 ± 3.30 (48.61%) | ‡‡‡ 19.5 ± 1.33 (47.30%) | ‡‡‡ 35.5 ± 2.14 (29.00%) | †††‡ 1210.71 ± 89.85 (19.06%) | †††‡ 1628.01 ± 111.92 (17.02%) |
Infected + PZQ + silymarin | ‡‡‡§§ 8.03 ± 1.69 (84.13%) | ‡‡‡§§ 20.60 ± 0.60 (67.09%) | ‡‡‡§ 20.5 ± 1.14 (44.59%) | ‡‡‡ 14.60 ± 1.72 (70.80%) | †‡‡‡ 964.74 ± 58.91 (35.51%) | †††‡‡‡ 1043.50 ± 58.45 (46.81%) |