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Figure 2 | Parasites & Vectors

Figure 2

From: Characterization of the biology and infectivity of Leishmania infantum viscerotropic and dermotropic strains isolated from HIV+ and HIV- patients in the murine model of visceral leishmaniasis

Figure 2

In vitro development of L. infantum strains. (A) Growth curves of Bibiano (light grey), E390M (dark grey), HL (black) and ST (white) L. infantum strains in NNN. Cultures were started with 106 promastigotes of each strain per mL and growth was followed for a week, with a daily counting of parasite numbers in a Neubauer chamber. (B) Parasite viability measured by flow cytometry as percentage of AnnV-/7AAD- cells. The mean of three independent experiments is plotted; bars represent SD. (C) Cell cycle analysis of HL during growth period. Data show means of one representative experiment of three independent assays. (D) Illustrative phenotype of L. infantum promastigotes with 4 days of culture in NNN captured by flow cytometry.

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Parasites & Vectors

ISSN: 1756-3305

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