Figure 6

Cell populations in spleens of naive and Leishmania -infected mice in the acute and chronic phases. (A), (F) Total cells were counted and stained for identification of major splenic populations by flow cytometry. (B), (G) CD4⁺ T cells. (C), (H) CD8⁺ T cells. (D), (I) B cells. (E), (J) Monocytes/macrophages. (A)-(E) Absolute number of cells per spleen. Data represent means ± SD of 3 to 5 animals per group of one experiment representative of two. (F)-(J) Fold modification of cell numbers in the infected mice in relation to naïve. Boxes and whiskers with 5–95 percentile and mean (showed by “+”) of 3 to 5 animals. Dashed and solid lines indicate a 2- or 4-fold modification, respectively, relative to naïve mice. One-way ANOVA followed by Dunnett’s multiple comparison test was performed to calculate statistically significant differences between naive and infected mice at 2 and 6 weeks after infection.