Molecular analysis of Baylisascaris columnaris revealed mitochondrial and nuclear polymorphisms

Parasites & Vectors

Table 3 Multi-locus types of Baylisascaris columnaris mitochondrial genes

Worm	Worm	Skunk	CO1 fragment						CO2 fragment				ML
isolates	gender	ID	59	61	79	91	229	259	66	117	280	480	type
K14, K16	F	N	T	G	C	A	G	G	G	A	C	G	I
K22, K24	M	V	T	G	C	A	G	G	G	A	C	G	I
K15, K10	F	N	T	G	C	A	G	G	G	A	C	G	II
K21	M	V	T	G	C	A	G	G	G	A	C	G	II
K17	F	V	C	G	T	A	A	G	G	G	T	T	III
K23	M	V	C	G	T	A	A	G	G	G	T	T	III
P24	F	P	C	G	T	A	A	G	G	G	T	T	III
P27	M	A	C	G	T	A	A	G	G	G	T	T	III
K19, 20	F	V	C	G	T	A	A	G	G	G	T	T	IV
B. procyonis isolates			T	A	T	G	A	A	A	A	T	T	-
B. transfuga isolates			T	A	T	G	T	G	G	A	T	G	-

Four different genotypes of Baylisascaris columnaris were found: two variants of mitochondrial genes CO1 and CO2, and two variants of nuclear genes ITS1-5.8S-ITS2 and 28S rDNA. These variants were linked two by two. Neither recombinations of mitochondrial type 1 and 2, nor recombinations of nuclear type 1 and 2 occurred in individual worms. No preference of any combination for worm gender was observed.

ISSN: 1756-3305