Differential cellular localization of GP82 and GP90 in parasites undergoing metacyclogenesis. Immunofluorescence was performed using exponentially growing epimastigotes, parasites attached to culture flasks at 48 h after nutritional stress and metacyclic trypomastigotes purified by DEAE-cellulose. Cells were fixed, permeabilized with 0.5% saponin, reacted with mAb 1G7 (A) or 3F6 (B) and incubated with secondary antibody Alexa Fluor 488. DAPI was used to stain nucleus (N) and kinetoplast (K). Scale bar = 10 μm.