Figure 2From: Expression and cellular trafficking of GP82 and GP90 glycoproteins during Trypanosoma cruzi metacyclogenesisDifferential cellular localization of GP82 and GP90 in parasites undergoing metacyclogenesis. Immunofluorescence was performed using exponentially growing epimastigotes, parasites attached to culture flasks at 48 h after nutritional stress and metacyclic trypomastigotes purified by DEAE-cellulose. Cells were fixed, permeabilized with 0.5% saponin, reacted with mAb 1G7 (A) or 3F6 (B) and incubated with secondary antibody Alexa Fluor 488. DAPI was used to stain nucleus (N) and kinetoplast (K). Scale bar = 10 μm.Back to article page