Figure 4
From: Identification of novel arthropod vector G protein-coupled receptors
GPCR Validation pipeline. Diamonds indicate input and output sequences. Rectangles represent the validation programs and filters. Arrows indicate the movement of sequences and are labeled with the number of sequences that passed from one validation step to the next. Numbers for sequences that were identified as something other than GPCRs are not shown. The first filter identifies sequences which are annotated as GPCRs in the database and removes any sequences which were annotated as something other than GPCRs or were identified by ScanPROSITE as having non-GPCR domains. The second filter uses a combination of the BLAST hits, I-TASSER results, and identification of GPCR domains by ScanPROSITE to categorize the final 52 predicted but unconfirmed sequences.