Table 1 Microsatellite markers of L. aethiopica developed and tested in this study
CO | REPEAT ARRAY | FORWARD PRIMER SEQUENCE (5′-3′) | REVERSE PRIMER SEQUENCE (5′-3′) | CHROMOSOME LOCATION | POLYMORPHISM |
|---|---|---|---|---|---|
1 | (CA)10 | TGAACAGTACGTCCAGGAGGA | GTGGTGGAAGAGGGAGAGAG | NA | P |
3 | (GT)20 | TCTTGCTTACGCGTGGACTA | ACGAGGCAGGAGGGAGAG | Lmj11 | NA |
4 | (CA)12 | TGCGCCAGTTTCTATGACTG | TGTGTTTGTGTGCGTGGATA | Lmx32, Ld33 | P |
5 | (GT)10 | TTATGATGTAGGCAGGGTAGAGT | GTATTGGGCAAAGGGACCTT | Lmj31, Ld31 | NA |
6 | (CA)29 | TGCTCATGGTGGATGTGC | GTCGTGTGGTCGTGTATGTG | Lmj31 | P |
7 | (CA)12 | TTCGTCGCTCTTGTCTCTTG | TAGTCCACGCGTAAGCAAGA | Lmj24 | NA |
8 | (CA)15 | TCCCTCTCTTCCTCTGTTCC | CAGATGCGCAAGAATCAAAA | Lmj, Ld15 | NA |
11 | (CA)16 | CTCCCTCTCTTCCTCTGTTCC | TCTCTCGGTGAGCCAGATG | Lmj, Ld15 | NA |
13 | (GT)16 | GGTCGTTGTGACGCCAGT | ACTGCGTTCTGTGTGTCTCCT | Lmj31 | NA |
15 | (GT)10 | CCACCACCACACTTTTTCGT | GATACCGGCTGATGATGAGG | NA | NA |
16 | (GT)13 | TCTTGCCTCTTTAATCTGTGTCC | GTTGTGGTGGTTGATCCGTT | Lmj34 | NA |
17 | (CA)28 | CTCACACATGGATGCAAAGC | CGTGTGTGTGTGCCATACAA | NA | NA |
18 | (GT)21 | GGAGGGGGAGGAGTCTAAAA | TGTACTGCCGAGATTGAACG | Lmj34, Li31,34, Ld8, 34 | NA |
19 | (GT)50, 30 | TCTTGCTTACGCGTGGACTA | TAGAGTTGATTGCACGTGGC | Lmj12 | NA |
20 | (GT)11 | TACTTCGCGCACCGTCTCT | TTGTCGGTCGTGACTACTGC | Lmj26 | NA |
21 | (GT)20 | GCTTACGCGTGGACTACCA | CTATCGAGGGTCGGCAAC | Lmj11,26 | NA |
22 | (GT)16 | GGCGCGATATCCACTTCTT | TGTAAGTGTGTGGGTGGGTG | Lmj18, Lin18 | P |
23 | (GT)12 | GTGGGTTTTTGAATTATGTCGTC | GTATTGGGCAAAGGGACCTT | Lmj31, Ld31, Lin31, Lmx30 | NA |
26 | (GT)15 | TCTTGCTTACGCGTGGACTA | TAGAGTTGATTGCACGTGGC | Lmj12, Ld12 | NA |
27 | (GT)40 | CGTACGTCTGTTGCCTCTGT | CACTACTCCGCAGAACCGTA | Ld26 | P |
28 | (GT)13 | CTCTTGCTTACGCGTGGACT | AGAGACAAGAGCGACGAAGC | Lmj24 | NA |
30 | (GT)16 | CCTTTGCCTTCTCCCTTTTT | GAGCTTGGCGTAATCATGGT | NA | NA |