Table 1 Primers used in this study
Region | Primer names | Sequence and 5′ position* | Amplicon length | |
|---|---|---|---|---|
Forward | Reverse | |||
Region 1 | Nimbus 1 | 5′-TTTTATGAGGTGTTTTAAGTGTTAGG-3′ (5′ position: 1132) | 5′-AAAAATTTCCCTTTATTCCAATAAC-3′ (5′ position: 1917) | 785 bp |
Region 2 | Nimbus 2 | 5′-TTGGATGTTAAAATTTTTGTTAGAA-3′ (5′ position: 2986) | 5′-AAAAAATATCCCTTAAACCCCATAA-3′ (5′ position: 3871) | 785 bp |
Region 3 | Nimbus 3 | 5′-ATTTTAGGGAATTGTAGGAGAGTTA-3′ (5′ position: 4802) | 5′-CTTATCAAACCCTTAAATATAAACC-3′ (5′ position: 5373) | 571 bp |