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Table 1 Primers used in this study

From: 5-methyl-cytosine and 5-hydroxy-methyl-cytosine in the genome of Biomphalaria glabrata, a snail intermediate host of Schistosoma mansoni

Region Primer names Sequence and 5′ position* Amplicon length
Forward Reverse
Region 1 Nimbus 1 5′-TTTTATGAGGTGTTTTAAGTGTTAGG-3′ (5′ position: 1132) 5′-AAAAATTTCCCTTTATTCCAATAAC-3′ (5′ position: 1917) 785 bp
Region 2 Nimbus 2 5′-TTGGATGTTAAAATTTTTGTTAGAA-3′ (5′ position: 2986) 5′-AAAAAATATCCCTTAAACCCCATAA-3′ (5′ position: 3871) 785 bp
Region 3 Nimbus 3 5′-ATTTTAGGGAATTGTAGGAGAGTTA-3′ (5′ position: 4802) 5′-CTTATCAAACCCTTAAATATAAACC-3′ (5′ position: 5373) 571 bp
  1. *position is given on the genbank sequence: EF413180.