Hydrolysis probe analysis fluorescence results for clades I and II of An. funestus s.s. from Zambia. Scatter plot analysis (a) and quantitative peaks (b) of A) clade I; B) clade II; C) negative control. Blue peaks are clade I and yellow peaks are clade II. Positive controls (arrows) from the Figure 2 experiment are D) clade I and E) clade II.