kDNA signatures of L. (V.) braziliensis obtained by LSSP-PCR and phenetic analysis. A: 1.8% High Resolution agarose gel electrophoresis showing representative genetic profiles derived from amplification of the 750 bp fragment from mucosal lesions of different patients. Lanes: M – Molecular marker (100 bp ladder); lanes 1–5: 22ML-N, 26ML-N, 29ML-N, 4MCL-O and 16MCL-O; lanes 6–12: 11MCL-N, 11MCL-C, 3MCL-N, 3MCL-C, 5MCL-N, 5MCL-O and 5MCL-C. B: UPGMA dendrogram showing the “Simple Matching” coefficients of similarity based on the genetic profiles obtained by LSSP-PCR. Lb, L. (V.) braziliensis reference strain (MHOM/BR/1975/M2903). MCL: mucocutaneous leishmaniasis; ML: mucosal leishmaniasis; DL: disseminated leishmaniasis; N: nasal; O: oral; C: cutaneous; C1-C3: different samples of cutaneous lesions collected at different moments; N1 and N2: different samples of mucosal nasal lesions collected at different moments.