Wolbachia -like peptides identified by mass spectrometry analysis of Onchocerca flexuosa lysate. In a recent proteomic analysis, two peptides detected in O. flexuosa adult worm lysate (shown in green) were mapped to an HlyD family secretion protein from the Wolbachia endosymbiont of Culex quinquefasciatus, accession number YP_001974912 . Polyclonal antibodies were raised against one of these peptides. The detected peptide, in its entirety, was predicted to be unfavorable for antibody production. Our selected epitope contains half of the detected peptide extended by six amino acids to the C-terminus to enhance solubility and immunogenic properties (underlined).