Figure 4

Western blot detection of putative peptides with antibodies to peptides corresponding to isotig12596, isotig21532, and Wolbachia HlyD in Onchocerca flexuosa adult worm lysate. Affinity-purified rabbit polyclonal antibodies against a peptide predicted from isotig12596 recognized a band at approximately 50kDa (lane 2). Antibodies raised against a peptide predicted from isotig21532 recognized two main bands at approximately 260 and 130 kDa (lane 4). Antibodies against a peptide from Wolbachia HlyD labeled two main bands at approximately 47 and 18 kDa (lane 6). Purified IgG from the pre-immune serum of the corresponding rabbits did not label these bands (lanes 1, 3 and 5). Approximate molecular masses (kDa) are shown on the left side of the blot.