Amino acid alignment of GDP-L-fucose transporters. The predicted amino acid sequence of schistosome GFT (Sm) is compared to GFTs of Caenorhabditis elegans (Ce), Drosophila melanogaster (Dm), Mus musculus (Mm) and humans (Hs) (accession numbers in Table 2). Alignment position is indicated above each block, and sequence length is reported to the right of each line. Positions of identity and positions exhibiting at least 80% conservation are highlighted in black and gray, respectively. The positions of ten well-conserved TMDs (underlined below sequences and alignment blocks) were determined using the Phobius transmembrane topology and signal peptide prediction server . Vector NTI Advance 11.0 software alignment settings: BLOSUM45 matrix, gap opening penalty = 12, gap extension penalty = 0.1, gap separation penalty range = 0, no residue-specific or hydrophobic residue gaps.