Luminescent multiplex viability assay for Trypanosoma brucei gambiense

Parasites & Vectors

Table 1 Sensitivity to hygromycin and lower detection limits of RLAS, EnduRen and CellTiter-Glo with the T.b. gambiense 348BT wild type and three RLuc transfected strains

Population	IC₅₀hygromycin^a	RLAS^b	EnduRen^c	CellTiter-Glo^d
wild type	< 0.12	n.a.	n.a.	218 ± 14
RLuc #2.1	7.5 ± 1.1	207 ± 22	991 ± 131	212 ± 9
RLuc #3.1	2.4 ± 0.7	810 ± 80	4804 ± 511	221 ±16
RLuc #3.2	4.2 ± 0.9	488 ± 50	3225 ± 423	215 ± 22

^a reported in μg ml^-1 ± SD, from 3 cultures and determined with fluorimetric assay for measurement of reduction of resazurin.
^b reported in number of cells ± SD, from 3 cultures and determined with RLAS, a luminescent assay for detection of Renilla luciferase activity in lysed cells.
^c reported in number of cells ± SD, from 3 cultures and determined with EnduRen, a luminescent assay for detection of Renilla luciferase activity in living cells.
^d reported in number of cells ± SD, from 3 cultures and determined with CellTiter-Glo, a luminescent assay for quantification of ATP.
n.a. = not applicable.

ISSN: 1756-3305