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Table 2 In vitro doubling time of the T.b. gambiense 348BT wild type and the recombinant RLuc #2.1 strain assessed in triplicate with EnduRen, CellTiter-Glo and by microscopy

From: Luminescent multiplex viability assay for Trypanosoma brucei gambiense

Population

Doubling timea

 

EnduRenb

CellTiter-Gloc

Microscopy

wild type

n.a.

12.7 ± 0.8

12.6 ± 0.9

RLuc #2.1

12.9 ± 0.7

12.4 ± 0.7

12.6 ± 0.5

  1. a reported in h-1 ± SD, from 3 cultures.
  2. b luminescent assay for measurement of Renilla luciferase activity in living cells.
  3. c luminescent assay for quantification of ATP.
  4. n.a. not applicable.
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Parasites & Vectors

ISSN: 1756-3305

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