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Figure 1 | Parasites & Vectors

Figure 1

From: Expression of trypsin modulating oostatic factor (TMOF) in an entomopathogenic fungus increases its virulence towards Anopheles gambiae and reduces fecundity in the target mosquito

Figure 1

Expression of Aea -TMOF increases Beauveria bassiana virulence to Anopheles gambiae mosquitoes. (A) Sugar fed and (B) blood fed adult female A. gambiae mosquitoes were challenged with Bb-Aa1 strain expressing Aea-TMOF or the wild type parent, by spraying with a suspension of 1x108 spores/ml and their survival was scored daily over the indicated period. (C) Three day old larvae were challenged by applying 5.5 x 108 spores (suspended in 50 μl oil) to the water surface and their survival was scored daily over the indicated period as described in the Methods section. Graphs represent percent survival as calculated by the Kaplan-Meier method for one representative experiment of each group. Statistical significance was calculated by the log rank test and survival curves were considered significantly different if P<0.05. (D) Six days after B. bassiana infection, larvae showed significant growth retardation compared to non-infected controls. Wild type B. bassiana (Bb-wt); TMOF-expressing strain (Bb-Aa1). Data shown are representative of at least three independent biological replicates using different batches of mosquitoes and conidia.

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Parasites & Vectors

ISSN: 1756-3305

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