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Table 1 LD 50 and LT 50 values of wild type and Aea -TMOF expressing Bb -Aa1 strain against Anopheles gambiae

From: Expression of trypsin modulating oostatic factor (TMOF) in an entomopathogenic fungus increases its virulence towards Anopheles gambiae and reduces fecundity in the target mosquito

LD50 and LT50 values of wild-type and TMOF expressing B. bassiana infections in Anopheles gambiae
Strain Mosquito stage LD50 (conidia/ml) LT50 (h)
Wild-type B. bassiana (sf) Adult 18.34 ± 2.86 x 107a 133.26 ± 12.79b
Bb: spAeaTMOF (sf) Adult 11.08 ± 2.79 x 107a (P=0.01) 113.23 ± 17.56b (P=0.009)
Wild-type B. bassiana (bf) Adult 18.00 ± 2.01 x 107c 115.04 ± 11.26d
Bb: spAeaTMOF (bf) Adult 11.31 ± 0.01 x 107c (P= 0.04) 86.48 ± 4.68d (P=0.01)
Wild-type B. bassiana Larvae 14.07 ± 1.24 x 107e 116.31 ± 13.69f
Bb: spAeaTMOF Larvae 11.32 ± 0.77 x 107e (P=0.01) 85.05 ± 14.66f (P=0.001)
  1. a,c LD50 calculated from the 76 h time point.
  2. e LD50 calculated from the 48 h time point using the Probit analysis.
  3. b,d Bioassays performed by spraying mosquitoes with a concentration of 1x108 spores/ml.
  4. f Larval bioassays performed using a concentration of 5x106 spores/ml.
  5. Statistical analysis was performed used the Student’s T-test and values were considered significant if P<0.05.