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Table 1 LD 50 and LT 50 values of wild type and Aea -TMOF expressing Bb -Aa1 strain against Anopheles gambiae

From: Expression of trypsin modulating oostatic factor (TMOF) in an entomopathogenic fungus increases its virulence towards Anopheles gambiae and reduces fecundity in the target mosquito

LD50 and LT50 values of wild-type and TMOF expressing B. bassiana infections in Anopheles gambiae


Mosquito stage

LD50 (conidia/ml)

LT50 (h)

Wild-type B. bassiana (sf)


18.34 ± 2.86 x 107a

133.26 ± 12.79b

Bb: spAeaTMOF (sf)


11.08 ± 2.79 x 107a (P=0.01)

113.23 ± 17.56b (P=0.009)

Wild-type B. bassiana (bf)


18.00 ± 2.01 x 107c

115.04 ± 11.26d

Bb: spAeaTMOF (bf)


11.31 ± 0.01 x 107c (P= 0.04)

86.48 ± 4.68d (P=0.01)

Wild-type B. bassiana


14.07 ± 1.24 x 107e

116.31 ± 13.69f

Bb: spAeaTMOF


11.32 ± 0.77 x 107e (P=0.01)

85.05 ± 14.66f (P=0.001)

  1. a,c LD50 calculated from the 76 h time point.
  2. e LD50 calculated from the 48 h time point using the Probit analysis.
  3. b,d Bioassays performed by spraying mosquitoes with a concentration of 1x108 spores/ml.
  4. f Larval bioassays performed using a concentration of 5x106 spores/ml.
  5. Statistical analysis was performed used the Student’s T-test and values were considered significant if P<0.05.