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Table 1 Primer sequences for the acetylcholinesterase-2 locus-based polymerase chain reaction assay

From: Mosquitoes established in Lhasa city, Tibet, China

1Primers

5′-3′sequences

Product size with B1246s (bp)

ACEpip

5′-GGA AAC AAC GAC GTA TGT ACT-3 ′

610

ACEpall

5′-ATG GTG GAG ACG CAT GAC G-3′

478

ACEquin

5′-CCT TCT TGA ATG GCT GTG GCA-3 ′

274

B1246s

5′-TGG AGC CTC CTC TTC ACG G-3 ′

 
  1. Primer sequences for the acetylcholinesterase-2 (ace-2) locus-based polymerase chain reaction assay.
  2. 1Primers of this multiplex PCR assay were designed based on the sequence differences in the acetylcholinesterase-2 (ace-2), developed by Smith, J. L. & Fonseca, D. M.