Table 1 DNA sequences of primer and probes
From: Ability to cause erythema migrans differs between Borrelia burgdorferi sensu lato isolates
Oligo name | Sequence (5’-3’) |
|---|---|
Duplex qPCR | |
OspA_F | AATATTTATTGGGAATAGGTCTAA |
OspA_R | CTTTGTCTTTTTCTTTRCTTACAAG |
FlaB-F | CAGAIAGAGGTTCTATACAIATTGAIATAGA |
FlaB-Rc/t | GTGCATTTGGTTAIATTGYGC |
OspA_probe | FAM-AAGCAAAATGTTAGCAGCCTTGA-BHQ-1™ |
FlaB-probe | JOE-CAACTIACAGAIGAAAXTAAIAGAATTGCTGCTGAICA |
IGS PCR | |
5S | GAGTTCGCGGGAGAGTAGGTTATTGCC |
23S | TCAGGGTACTTAGATGGTTCACTTCC |
OspC PCR | |
OC6 | AAAGAATACATTAAGTGCGATATT |
OC602 | GGGCTTGTAAGCTCTTTAACTG |