Figure 3

Functional study of miRNA intervention after miR-mimic and inhibitor injection in Aedes albopictus developmental stages. [A] Hatching rate of embryo after miR-286b injection with mimic, inhibitor, negative control and normal un-injected embryos. * A significant difference (p < 0.001) of inhibitor group with the negative control group in hatching rate of eggs into larvae (statistically analyzed by ANOVA, LSD). The error bar shows the SD of values from three independent experiments, and is statistically evaluated by ANOVA (F = 191.557 & p < 0.001). [B] Daily hatching after embryo injection with miR-286b mimic, inhibitor, negative control and normal un-injected embryo. The 1^st hatching day is 2 days after the microinjection. The error bar shows the SD of values from three independent experiments. [C] Eclosion rate in larvae after miR-2942 injection with mimic, inhibitor, negative control and normal un-injected embryo analyzed statistically by ANOVA (F = 38.193 & p < 0.001). * A statistically significant difference (p < 0.001) of inhibitor group with the negative control group and other two groups (statistically analyzed by ANOVA, LSD). A, B, C indicates statistically significant difference (p < 0.001) between the groups (statistically analyzed by ANOVA, LSD). The error bar shows the SD of values from three independent experiments. [D] Fecundity rate after miR-1891 injection. Result analyzed by ANOVA (F = 58.886 and p < 0.001). *A significant decrease in mean eggs per female in inhibitor group (p < 0.001) compared with negative control group (statistically analyzed by ANOVA, LSD) and all other groups observed. The error bar shows the SD of values from three independent experiments.