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Table 2 Key factors for good (20–60%) excystation yields

From: Development of an in vitro drug sensitivity assay based on newly excysted larvae of Echinostoma caproni

Excystation factor

Optimal values

Cyst storage time

0–4 weeks

TBC medium:

 

     Trypsin concentration

1–5 mg/ml

     Bile salts concentration

3–7 mg/ml

     L-cysteine concentration

2–8 mg/ml

     Amount of TBC medium

≥ 3 ml

     Amount of cysts exposed to medium

No optimum

pH of TBC medium

7.8–8.2

Incubation temperature

39–41°C

Incubation time

1–2.5 hrs