Excystation factor | Optimal values |
---|---|
Cyst storage time | 0–4 weeks |
TBC medium: | |
Trypsin concentration | 1–5 mg/ml |
Bile salts concentration | 3–7 mg/ml |
L-cysteine concentration | 2–8 mg/ml |
Amount of TBC medium | ≥ 3 ml |
Amount of cysts exposed to medium | No optimum |
pH of TBC medium | 7.8–8.2 |
Incubation temperature | 39–41°C |
Incubation time | 1–2.5 hrs |