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Figure 1 | Parasites & Vectors

Figure 1

From: Molecular characterization of Trichinella spiralis aminopeptidase and its potential as a novel vaccine candidate antigen against trichinellosis in BALB/c mice

Figure 1

Identification of recombinant TsAP protein. (A) SDS-PAGE analysis of recombinant TsAP protein. M: protein molecular weight marker; 1: the lysis of the induced recombinant bacteria after ultrasonication; 2: TsAP inclusion body dissolved in 8 M urea; 3: recombinant TsAP protein purified by GST Sefinose Resin. (B) Western-blot analysis of recombinant TsAP protein antigenicity. The T. spiralis crude antigens (lane 1), ES antigens (lane 2), and recombinant TsAP protein (lane 3) were recognized by sera of mice infected with T. spiralis at 30 dpi. The native TsAP protein in crude antigens (lane 4) and recombinant TsAP protein (lane 6) were recognized by anti-TsAP serum, but the ES antigens (lane 5) were not recognized by anti-TsAP serum. The T. spiralis crude antigens (lane 7), ES antigens (lane 8), and recombinant TsAP protein (lane 9) were not recognized by sera of normal mice.

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Parasites & Vectors

ISSN: 1756-3305

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