Figure 4

Expression of TsAP at different developmental stages and immunolocalization in T. spiralis. A–H: The results of IFT with whole parasite of T. spiralis different stages reacted with anti-TsAP serum. The notable immunostaining is found in the body of AW at 3 dpi (A), NBL (B), PEL at 19 dpi (C) and ML at 42 dpi (D). The ML at 42 dpi reacted with serum of mice infected T. spiralis at 30 dpi (E) as a positive control; ML at 42 dpi did not show recognition by normal serum (F) as a negative control. G–L: The results of IFT with the sections of skeletal muscles of infected mice reacted with anti-TsAP serum. The immunostaining is seen at the cuticle and internal organs of PEL at 19 dpi (G) and ML at 42 dpi (H and I). The ML at 42 dpi reacted with anti-ES serum (J) and serum of mice infected T. spiralis at 30 dpi (K) as a positive control, ML at 42 dpi did not did not show recognition by normal mouse serum (L) as a negative control.