EhCP112 silencing. Trophozoites were incubated at different times with 50 μg/ml of the siRNA 1 via the soaking method and the silencing of EhCP112 was analyzed by Western blot assays. (A) Western blot. Total extracts obtained from non-treated trophozoites (Ctl+), trophozoites incubated with a double-strand RNA with non-related sequence (NRS) and trophozoites incubated with the siRNA 1 at different times (16, 24 and 36 h) were analyzed by Western blot using the antibodies against rEhCP112. As internal control, membranes were also incubated with an anti-actin antibody. (B) Relative expression of EhCP112. The bands recognized by antibodies were analyzed by densitometry. The EhCP112 expression was normalized to actin expression and the value obtained in non-treated trophozoites was taken as 100% of relative expression. Asterisks: P < 0.001.