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Table 1 Known and novel microRNAs (miRNAs) identified from the pancreatic fluke Eurytrema pancreaticum

From: Identification and characterization of microRNAs in the pancreatic fluke Eurytrema pancreaticum

Name

Location at genomea

mfeb

Countc

locationd

Sequencee

Known miRNA

sja-bantamf

SJC_S000254:283545:283639:+

−27.5

3488

3p

TGAGATCGCGATTAAAGCTGG

sja-let-7

SJC_S005824:19533:19622:+

−33.1

50

5p

GGAGGTAGTTCGTTGTGTGGT

sja-miR-2a

SJC_S000054:242652:242727:-

−34.2

4406

3p

TCACAGCCAGTATTGATGAAC

sja-miR-2b

SJC_S000054:242557:242635:-

−33.6

500

3p

TATCACAGCCCTGCTTGGGACAC

sja-miR-2c

SJC_S000102:360645:360725:+

−28.42

62

3p

TATCACAGCCGTGCTTAAGGGC

sja-miR-2d

SJC_S000102:360534:360627:+

−33.1

727

3p

TATCACAGTCCTGCTTAGGTGACG

sja-miR-2e

SJC_S000054:242457:242536:-

−24.7

1721

3p

TATCACAGTCCAAGCTTTGG

sja-miR-71

SJC_S000054:242738:242817:-

−33.01

19048

5p

TGAAAGACGATGGTAGTGAGA

sja-miR-71b

SJC_S000102:360305:360397:+

−36.2

144949

5p

TGAAAGACTTGAGTAGTGAGACGC

sja-miR-124

SJC_S000254:113694:113791:+

−29.8

32

3p

TAAGGCACGCGGTGAATGTCA

sja-miR-8

SJC_S001790:88898:88974:+

−31.5

12

3p

TAATACTGTTAGGTAAAGATGC

sja-miR-2162

SJC_S000471:21744:21822:-

−37.9

13

3p

TATTATGCAACGTTTCACTCT

sja-miR-10g

SJC_S000052:310019:310093:+

−23.4

2698/11

5p/3p

AACCCTGTAGACCCGAGTTTG/AAATTCGAGTCTATAAGGA

Novel miRNA

Epa-miR-01

SJC_S000054:242558:242634:-

−31.1

97

3p

TATCACAGCCCTGCTTGGGACA

Epa-miR-02

SJC_S000057:543062:543147:-

−20.4

7

5p

AGAAGGCTGCGTGTTCGGATC

Epa-miR-03

SJC_S000065:176156:176237:+

−22

7

5p

CTGTCTTCTCTCTCATGTTC

Epa-miR-04

SJC_S000083:92590:92686:+

−27.7

16

3p

GTTTGAGACTCCGAATGATG

Epa-miR-05

SJC_S000102:360305:360397:+

−36.2

151

5p

TGAAAGACTTGAGTAGTGAG

Epa-miR-06

SJC_S000102:360535:360626:+

−33.1

111

3p

TATCACAGTCCTGCTTAGGTGAC

Epa-miR-07

SJC_S000102:360645:360725:+

−28.42

62

3p

TATCACAGCCGTGCTTAAGGGC

Epa-miR-08

SJC_S000115:155214:155289:-

−18.2

138

3p

GCTATCTCGTCGATACTGGC

Epa-miR-09

SJC_S000254:113694:113791:+

−29.8

32

3p

TAAGGCACGCGGTGAATGTCA

Epa-miR-10

SJC_S000254:283542:283641:+

−30.7

176

3p

TGAGATCGCGATTAAAGCTGGTT

Epa-miR-11

SJC_S000471:21744:21822:-

−37.9

13

3p

TATTATGCAACGTTTCACTCT

Epa-miR-12

SJC_S001790:88898:88974:+

−31.5

12

3p

TAATACTGTTAGGTAAAGATGC

Epa-miR-13

SJC_S005824:19533:19622:+

−33.1

50

5p

GGAGGTAGTTCGTTGTGTGGT

Epa-miR-14g

SJC_S008424:1:71:+

−30.1

33/8

5p/3p

GACGGGGTGGCCGAGTGGTT/CCATTGGGGTTTCCCCGCGT

  1. Note: a location of mature miRNAs at the reference genome of Schistosoma japonicum; b energy of stem-loop structure of miRNA precursors, with unit as Kcal/mol; c sequencing count; d location of a mature miRNA at the 3p or 5p arm of its precursor; e sequence of mature miRNA; f Known miRNAs matched perfectly with that from S. japonicum deposited in the miRBase database; g the only two miRNAs (miR-10, Epa-miR-14) that had mature miRNAs both detected at the 5p and 3p arms of their precursors in the E. pancreaticum. Blue: the largest family members of miR-2.
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Parasites & Vectors

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