Real-time PCR assay in differentiating Entamoeba histolytica, Entamoeba dispar, and Entamoeba moshkovskii infections in Orang Asli settlements in Malaysia

Table 2 Prevalence of Entamoeba infection based on microscopy, nested PCR, and real-time PCR assays

Location	No.	Microscopy		Nested PCR		Real-time PCR
Location	Examined	n	%	n	%	n	%
Kampung Gurney	48	5	10.4	4	80	5	100
Kampung Bumbun	50	14	28	12	85.7	14	100
Kampung Pangsun	55	10	18.2	10	100	10	100
Kampung Sungai Minyak	29	2	6.9	2	100	2	100
Kampung Kemensah	54	2	3.7	2	100	1	50
SK Pos Piah	30	19	63.3	14	73.7	16	84.2
Kampung Teras Pos Piah	68	13	19.1	8	61.5	8	61.5
Total	334	65	19.5	52	80	56	86.2

ISSN: 1756-3305