Figure 1From: Prostaglandin E2 in tick saliva regulates macrophage cell migration and cytokine profileTick saliva causes PGE 2 secretion by macrophages. Cells were treated with vehicle (PBS) or saliva for 18 h. Increasing the dose of saliva resulted in a higher level of macrophage PGE2 secretion reaching significance (p < 0.05) at 3.6 μg protein/ml, (*) p < 0.01 when compared to vehicle control. The PGE2 concentration of the pooled saliva used for all experiments was 352 ± 9 ng/ml, which was diluted to deliver 3.6 μg protein/ml. If still present in the sample, the PGE2 from the pooled saliva would only account for approximately 1.2% of the total PGE2. Data are means ± SEM, n = 3 assayed in duplicate.Back to article page