Figure 2

Saliva-induced effects on macrophage and fibroblast migration are PGE ₂ receptor antagonist-sensitive. (A) Macrophages were pretreated for 30 min with vehicle (DMSO), saliva (2.4 μg protein/ml), or PGE₂ (1 μM) in the absence or presence of the PGE₂ receptor antagonist AH 6809 (10 μM), and then incubated for 4 h. PDGF increased the number of cells migrating, an effect enhanced by saliva and PGE_2, (**) p < 0.001 and (*) p < 0.01 when compared to PDGF treatment only. These effects were reversed by AH 6809, (**) p < 0.001 when saliva treatment was compared to saliva + AH 6809 and when PGE₂ was compared to PGE₂ + AH 6809. (B) Fibroblasts were pretreated for 30 min with vehicle (DMSO), saliva (2.4 μg protein/ml), PGE₂ (1 μM), or conditioned medium from macrophages treated with saliva for 18 h in the absence or presence of the AH 6809 (10 μM) using PDGF as the chemoattractant. After 4 h, saliva, PGE₂, and saliva-treated macrophage conditioned medium decreased fibroblast migration (CM), (**) p < 0.001 when compared to PDGF treatment only. The effects of saliva were partially reversed by AH 6809; however, the receptor antagonist fully restored the migration of cells treated with conditioned medium of saliva-treated macrophages (CM) similar to that of PGE₂ in the presence of AH 6809, (**) p < 0.001 when PGE₂ was compared to PGE₂ + AH 6809 and when CM was compared to CM + AH 6809, (#) p < 0.05 when saliva was compared to saliva + AH 6809. Data are reported as the % control values and are means ± SEM, n = 3.