Figure 3

Saliva-induced decreases on CCL5, sTNFRI, and TNF-α secretion by macrophages are PGE ₂ receptor antagonist-sensitive. Cells were treated with vehicle (DMSO), saliva (2.4 μg protein/ml), AH 6809 (10 μM), or saliva + AH 6809 for 18 h. (A) Saliva reduced the relative expression levels of secreted CCL5, soluble TNF Receptor I (sTNFRI), and TNF-α which was reversed by AH 6809. (B) Image of blots exposed to film for 2.5 min corresponding to the treatments in (A), cylinder = CCL5, oval = sTNFRI, and rectangle = TNF-α, (**) p < 0.001 when compared to vehicle control and when saliva was compared to saliva + AH 6809. Data are means ± SEM normalized to the vehicle treated array reported as relative expression levels determined by densitometry for 1 and 2.5 min exposure times.