The Anopheles community and the role of Anopheles minimus on malaria transmission on the China-Myanmar border

Parasites & Vectors

Table 1 Primers used for multiplex PCR and nested PCR

Species	Primer name	Sequences (5′ to 3′)	Amplification size (bp)	Sources
Forward primer for Anopheles	ANF	ATCACTCGGCTCATGGATCG		Phuc et al. (2003)
An. minimus	MAR	GGGCGCCATGTAGTTAGAGTTG	184
An. harrisoni	MCR	GGTTGCCCACTCAATACGGGTG	509
Reverse primer for animal	UNR	GGTTGTCCTCCAATTCATGTTA		Kent et al. (2005)
Human	HUF	GGCTTACTTCTCTTCATTCTCTCCT	335
Cow	COF	CATCGGCACAAATTTAGTCG	561
Pig	PIF	CCTCGCAGCCGTACATCTC	453
Dog	DOF	GGAATTGTACTATTATTCGCAACCAT	680
P. falciparum	PFF	TTAAACTGGTTTGGGAAAACCAAATATATT	205	Snounou et al. (1993)
	PFR	ACACAATGAACTCAATCATGACTACCCGTC
P. vivax	PVF	CGCTTCTAGCTTAATCCACATAACTGATAC	121
	PVR	ACTTCCAAGCCGAAGCAAAGAAAGTCCTTA

ISSN: 1756-3305