Figure 1

Specificity of capture probes and target bacteria detected by the DNA microarray. (A) Fluorescence intensity at 635 nm (F634Mean-B635) of specific capture probes coupled to target Cy5-labeled amplicons. (B) Fluorescence intensity expressed as a percentage of the fluorescence intensity of the capture probe in relative to 100% of the fluorescence intensity of the M probe (positive control). All capture probes are listed in Table 1. The targeted bacteria were (CNe) Candidatus Neoehrlichia mikurensis, (Rc) Rickettsia spp., (Cb) Coxiella spp., (Bsp) Borrelia spp., (An) Anaplasma spp., (Fr) Francisella spp.