Alteration of miRNA expression profiles in serum of infected mice with schistosomiasis japonica. Mice were infected percutaneously with 16 S. japonicum cercariae or remained uninfected (normal group, blank square). Half of the infected mice were treated with PZQ for 3 days (PZQ treated group, black triangle) and half were not treated (untreated group, black square). All the mice were sacrificed at 72 days after infection to harvest the serum and liver samples. (A) Hydroxyproline content of liver samples of mice from the three groups was detected. Expression levels of serum miR-223 (B), miR-122 (C), miR-34a (D), miR-199a-5p (E) miR-199a-3p (F), and miR-146b (G) were detected in the three groups of mice. The data shown are measurements from individual mice. The bars designated the means for each group.