Table 1 Oligonucleotide probes used in the Luminex suspension array for the detection and species identification of ovine piroplasms
Probe | Sequence (5’- 3’) | Size (bp) | nmol | References |
|---|---|---|---|---|
Catch-all TB | TAATGGTTAATAGGA(A/G)C(A/G)GTTG | 22 | 1 | [11] |
Babesia ovis | CGCGGCCTTTGCGTTACTTT | 20 | 0.5 | This work |
Babesia motasi | TCCGTTATTGGAGTATTGCG | 20 | 0.5 | This work |
Theileria ovis | TTTTGCTCCTTTACGAGTCTTTGC | 24 | 0.5 | [1] |
Theileria luwenshuni/OT1 | ATCTTCTTTTTGATGAGTTGGTGT | 24 | 0.5 | [1] |
Theileria sp. OT3 | ATTTTCTCTTTTTATATGAGTTTT | 23 | 0.5 | [1] |
Theileria annulata / lestoquardi | GGGTCTGTGCATGTGGCTTTT | 20 | 0.5 | [8] |
IAC | GCATCGGTTACAAGAACGCA | 20 | 0.5 | [8] |