Locus
|
Primer Sequence 5′ -- > 3′
|
Repeat motif
|
TA
|
Size (bp)
|
N
|
k
|
---|
Shae_01
|
F: †GCATCCAATTTCGTACAC
|
AAT
|
TD60
|
257 - 307
|
65
|
12
|
|
R: *CCACATTAGGCCAACAAG
| | | | | |
Shae_02
|
F: *TTAGTGTGTTTGGCTTCAAC
|
AAT
|
TD60
|
183 – 225
|
69
|
9
|
|
R: †CCTCGAATGAAATCCTGAC
| | | | | |
Shae_03
|
F: †GCTGAGCTTGAGATTG
|
AAT
|
TD55
|
291 – 334
|
62
|
15
|
|
R: *CTTCTGTCCCATCGATACC
| | | | | |
Shae_04
|
F: †CCCATCGCTGATATTAAAG
|
AAT
|
TD65
|
289 – 325
|
70
|
10
|
|
R: *TCTAGTCGTCTTGGGATCC
| | | | | |
Shae_05
|
F: *TGTGCACAAGAAAGATTAAATG
|
AAT
|
TD65
|
288 – 319
|
68
|
10
|
|
R: †ACGACAATGTTGCAAGTTC
| | | | | |
Shae_06
|
F: †GGTGGATTACGCAATAG
|
AC
|
TD65
|
333 – 347
|
67
|
8
|
|
R: *TTTAATCAACCGGGTGTC
| | | | | |
Shae_07
|
F: *TCCAAGCACCATTATCAAG
|
AAT
|
TD65
|
315 – 330
|
66
|
6
|
|
R: †ACGGAAACTTGTTGAAATG
| | | | | |
Shae_08
|
F: *CTAAACTGGCAAGATTTC
|
AAT
|
TD65
|
299 – 330
|
68
|
11
|
|
R: †CAACGTGCCTTTATTTC
| | | | | |
Shae_09
|
F: †GGGATGTATGCAGACTTG
|
AAT
|
TD65
|
213 – 256
|
68
|
13
|
|
R: *TTGTTTGGCTGCAGTAAC
| | | | | |
Shae_10
|
F: †CGCATGTCATACCTATCTCC
|
AAT
|
TD65
|
198 – 213
|
69
|
6
|
|
R: *GCTTATCAGGCCTATCTCC
| | | | | |
Shae_11
|
F: *TTGGTTTAGAAATTACATCACC
|
ATC
|
TD65
|
207 – 222
|
66
|
5
|
|
R: †CCAACAATATTAATGGACAGC
| | | | | |
Shae_12
|
F: †CGTCTTAGTGAGCCAGATG
|
AAC
|
TD65
|
257 – 278
|
68
|
6
|
|
R: *CTCGTGGACATCATCAG
| | | | | |
Shae_13
|
F: *GAGCAGCTATTTCGTATCG
|
AAT
|
TD60
|
183 – 225
|
69
|
12
|
|
R: †ACCGTGGACAGTTCATCAG
| | | | | |
Shae_14
|
F: *GTCCTCCTTCCCTCTTTG
|
ACTC
|
TD65
|
210 – 254
|
67
|
10
|
|
R: †CACATTCGTCCTAGATATCG
| | | | | |
Shae_15
|
F: *CTTTCAGTAGGATTTGTTG
|
ATC
|
TD65
|
300 – 322
|
67
|
9
|
|
R: †CGACGTCAAGCACTGTAC
| | | | | |
- * indicates CAG tag (5′- CAGTCGGGCGTCATCA-3′).
- † indicates pigtail (5′- GTTT-3′).
- The annealing temperature (T
A
) where TD65, TD60, and TD55 indicates touchdown protocols with a highest annealing temperature of 65°C, 60°C, and 55°C, respectively; size indicates the range of sequenced alleles in base pairs plus the length of the CAG tag; the number of individuals genotyped out of 72 is N; k is the number of alleles observed.