Gel images for blood meal analysis (a) and Leishmania detection PCR (b). a) Agarose gel (1.5 %) image of the blood meal analysis using the universal vertebrate cytb primers; (M- marker lane, 1- PCR done with human DNA, 2- PCR done with cattle DNA, 3-8 in row1 and 1-12 in row2 the PCR done with sandfly extractions). b) Semi nested PCR amplified products of mini circle kinetoplastid DNA: (M- Marker [100bp] Lanes 1-8 in row1 and 1-7 in row 2 are female Ph. (Eup.) argentipes sibling species A from Hambantota, lane 8- negative control; male Ph. (Eup.) argentipes sibling species A extract from Hambantota ). c) ITS1 PCR amplified products in 1.5% agarose gel (M- 100 bp marker, 1 and 9- negative controls [1- female sibling species B from Delft, 9- male sibling species A from Hambantota, all the other lanes are sibling species A female extracts from Hambantota).