Figure 3

Cell surface markers in uninfected macrophages, Wh3-Mφ and Wh6-Mφ. (A) After three days postinfection, mice were euthanized and PECs were harvested. Three hours later adherent peritoneal macrophages were collected and stained for F4/80, CD80, CD86 and MHC class II. (B) As in (A), Wh3-Mφ and Wh6-Mφ were stained for F4/80, PD-L1, PD-L2 and CD206. Flow cytometry graphs show histograms of F4/80-gated macrophages (dotted lines, the isotype control; solid lines, uninfected macrophages; heavy lines, Wh3-Mφ; tinted and hairline, Wh6-Mφ). (C, D and E) MFIs of F4/80 (C), CD80, CD86 and MHC class II molecule (D), PD-L1, PD-L2 and CD206 (E) on uninfected macrophages, Wh3-Mφ or Wh6-Mφ are displayed (^#p>0.05, ^*p<0.05, ^**p<0.01,^***p<0.001, Wh3-Mφ or Wh6-Mφ vs uninfected Mφ). Data are representative of five independent experiments.