Microbial communities and symbionts in the hard tick Haemaphysalis longicornis (Acari: Ixodidae) from north China

Table 1 Oligonucleotide primers used for PCR amplification and sequencing

Primer name	Genera or Species	Target gene	Nucleotide sequence (5′-3′)	Annealing temperature (°C)	Approx product size (bp)	Reference
CLS F	Coxiella	16S rRNA	CACGTAGGAATCTACCTTGTAG	55	90	55
CLS R			CGTTTTGTTCCGAAGAAATTAT
ALS 82 F	Arsenophonu	16S rRNA	AGGGAGCTTGCTTCCTGGCCGG	59	130	55
ALS 198R			CGAAGGTGTGAGGCCTAATGG
Rickettsia 354 F	Rickettsia	16S rRNA	CAGCAATACCGAGTGAGTGATGAAG	56	350	69
Rickettsia 647R			AGCGTCAGTTGTAGCCCAGATG
NCLS F	NCLS	16S rRNA	TCCCTGGCGGCGAGTGG	55	110	This study
NCLS R			CGTATTAGAGATTAGAGAAACC
Rr 190.70p	Rickettsia	rompA	ATGGCGAATATTTCTCCAAAA	48	540	57
Rr 190.602n			GTTCCGTTAATGGCAGCATCT

ISSN: 1756-3305