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Table 2 Comparative alignment and size differentials of the 13 unique ITS2 haplotypes detected in 192 specimens of An. oswaldoi s.l. from Brazil (BR, n = 27), Colombia (CO, n = 154), Peru (PE, n = 1) and Trinidad and Tobago (TR, n = 10)

From: Systematics of the Oswaldoi Complex (Anopheles, Nyssorhynchus) in South America

    2 2 3 3 3 3 3 3 3 3 3 3 3 3 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4
    0 5 1 3 3 3 4 4 4 6 6 6 6 6 0 0 0 1 1 1 1 1 5 6 6 6 7 7 7 7 8 9
Haplptypes n Spicemen origen 7 9 0 2 7 8 2 4 5 5 6 7 8 9 4 8 9 2 6 7 8 9 5 1 7 8 0 3 4 7 6 4
HI 12 Espirito Santo/São BR A C A A - - A - - A - - A C C A G C - - - - C A - - G - - G A C
HII 1 Mato Grosso, BR A C T C - - A - - A C C A T C A G A A G A A C A - - G G C G A A
HIII 2 Amazonas, BR & Amazonas, CO G C T C - - A - - A T C A T C A G A A G A A C A - - G G C G G A
HIV 4 Mato Grosso, BR G C T C - - A - - A C C A T C A G A A G A A C A - - G G C G G A
HV 3 Mato Grosso, BR G C T C - - A - - A C C A T C A G A A G A A C A - - G G C G A A
HVI 1 Mato Grosso, BR G C T A - - A A G A C C A T C A G A A G A A C G - - G G C G A C
HVII 3 Mato Grosso, BR G C T A - - A A G A T C A T C A G A A G A A C A - - G G C G G A
HVIII 1 Mato Grosso, BR G C T A - - A A G A C C A T C A G A A G A A C A - - G G C G A C
HIX 1 Mato Grosso, BR G C T A - - A A G A T C A T C A G A A G A A C A - - C G C G A C
HX 29 Antoquia & Norte de Santander, CO A T T C C A A - - - - - - - A G G C - - - - C A T C C G C A A C
HXI 10 St Andrews, TR A T T C C A A - - - - - - - A G G C - - - - C G T C G G C A A C
XHII 124 Putumayo & Caqueta, CO A T T A C A A - - - - - - - A G G C - - - - C G - - G G C G A C
HXIII 1 Madre de Dios, PE A T A A - - G - - G C C A T C - - A A G A A T A - -   G C G G A
  1. Following primer trim, the ITS2 fragments varied in length from 530–540 bp. In the final 544-bp alignment, 32 sites were found to be variable. H1 includes specimens of the lectotype series of An. oswaldoi s.s [12].