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Table 2 Comparative alignment and size differentials of the 13 unique ITS2 haplotypes detected in 192 specimens of An. oswaldoi s.l. from Brazil (BR, n = 27), Colombia (CO, n = 154), Peru (PE, n = 1) and Trinidad and Tobago (TR, n = 10)

From: Systematics of the Oswaldoi Complex (Anopheles, Nyssorhynchus) in South America

   

2

2

3

3

3

3

3

3

3

3

3

3

3

3

4

4

4

4

4

4

4

4

4

4

4

4

4

4

4

4

4

4

   

0

5

1

3

3

3

4

4

4

6

6

6

6

6

0

0

0

1

1

1

1

1

5

6

6

6

7

7

7

7

8

9

Haplptypes

n

Spicemen origen

7

9

0

2

7

8

2

4

5

5

6

7

8

9

4

8

9

2

6

7

8

9

5

1

7

8

0

3

4

7

6

4

HI

12

Espirito Santo/São BR

A

C

A

A

-

-

A

-

-

A

-

-

A

C

C

A

G

C

-

-

-

-

C

A

-

-

G

-

-

G

A

C

HII

1

Mato Grosso, BR

A

C

T

C

-

-

A

-

-

A

C

C

A

T

C

A

G

A

A

G

A

A

C

A

-

-

G

G

C

G

A

A

HIII

2

Amazonas, BR & Amazonas, CO

G

C

T

C

-

-

A

-

-

A

T

C

A

T

C

A

G

A

A

G

A

A

C

A

-

-

G

G

C

G

G

A

HIV

4

Mato Grosso, BR

G

C

T

C

-

-

A

-

-

A

C

C

A

T

C

A

G

A

A

G

A

A

C

A

-

-

G

G

C

G

G

A

HV

3

Mato Grosso, BR

G

C

T

C

-

-

A

-

-

A

C

C

A

T

C

A

G

A

A

G

A

A

C

A

-

-

G

G

C

G

A

A

HVI

1

Mato Grosso, BR

G

C

T

A

-

-

A

A

G

A

C

C

A

T

C

A

G

A

A

G

A

A

C

G

-

-

G

G

C

G

A

C

HVII

3

Mato Grosso, BR

G

C

T

A

-

-

A

A

G

A

T

C

A

T

C

A

G

A

A

G

A

A

C

A

-

-

G

G

C

G

G

A

HVIII

1

Mato Grosso, BR

G

C

T

A

-

-

A

A

G

A

C

C

A

T

C

A

G

A

A

G

A

A

C

A

-

-

G

G

C

G

A

C

HIX

1

Mato Grosso, BR

G

C

T

A

-

-

A

A

G

A

T

C

A

T

C

A

G

A

A

G

A

A

C

A

-

-

C

G

C

G

A

C

HX

29

Antoquia & Norte de Santander, CO

A

T

T

C

C

A

A

-

-

-

-

-

-

-

A

G

G

C

-

-

-

-

C

A

T

C

C

G

C

A

A

C

HXI

10

St Andrews, TR

A

T

T

C

C

A

A

-

-

-

-

-

-

-

A

G

G

C

-

-

-

-

C

G

T

C

G

G

C

A

A

C

XHII

124

Putumayo & Caqueta, CO

A

T

T

A

C

A

A

-

-

-

-

-

-

-

A

G

G

C

-

-

-

-

C

G

-

-

G

G

C

G

A

C

HXIII

1

Madre de Dios, PE

A

T

A

A

-

-

G

-

-

G

C

C

A

T

C

-

-

A

A

G

A

A

T

A

-

-

 

G

C

G

G

A

  1. Following primer trim, the ITS2 fragments varied in length from 530–540 bp. In the final 544-bp alignment, 32 sites were found to be variable. H1 includes specimens of the lectotype series of An. oswaldoi s.s [12].
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