Expression of SjCyP18 in different stages of S. japonicum . A RT-PCR analysis of cDNAs from different stages of S. japonicum including cercariae (lane 1), male adult (lane 2), female adult (lane 3) and egg (lane 4). B Immunofluorescence staining of SjCyP18 in cercariae, female adult worms, male adult worms and eggs of S. japonicum. Parasite sections were reacted with anti-serum produced in mice against recombinant SjCyP18 and was then detected with Alexa Fluor 488 conjugated goat anti-mouse IgG. Preimmune normal serum and anti-TSP2-serum were used as negative and positive controls respectively.