Analysis of anti-SjCyP18 IgG in S. japonicum infected patients and anti-SjCyP18 IgG and its subclasses in infected mice. A Sera from 10 S. japonicum infected patients who were positive by Kato-Katz test and from 5 normal subjects were measured for anti-SjCyP18 or anti-SEA IgG by ELISA. The significance test was analyzed by unpaired t-test with * as p < 0.05 and ** as p < 0.01. B The time course of anti-SjCyP18 IgG in S. japonicum infected mice, showing the mean ± SEM from 4 mice, representative of two separate experiments. One-way ANOVA was used to test the significance on the levels of day 42 with all the other time points with *** as p < 0.001. C Mouse sera from 42d Sj infected mice were measured for anti-SjCyP18 or anti-SEA IgG1 or IgG2c by ELISA as described in the methods. The results shown on IgG subclasses were the mean value of 4 mice and a representative of 5 separately performed experiments. Two-way ANOVA was used to test the significances of differences with *** as p < 0.001.