Induction of Th2 response by footpad injection of SjCyP18. Draining popliteal lymph node cells were isolated 4 days after SEA (A) or SjCyP18 (B) footpad injection. The IL-4 and IFN-γ concentration in the supernatants from lymphocytes reactivated with anti-CD3 and anti-CD28 antibodies were measured by sandwich ELISA as described in Methods. Results shown were representative results of five different experiments. Student’s t test was used to analyze the significance with ** as p < 0.01, *** as p < 0.001.