Distribution of the N. caninum quantified proteins after secretion stimulus. A, Distribution of the proteins in the discharged tachyzoite (ratio discharged/control). Up-regulated proteins are in blue and down-regulated proteins are in pink. B, Comparison of the distribution of proteins identified from N. caninum secreted fraction (ESA) to the proteins from the total extracts (TE). ESA proteins are in purple, TE proteins are in light green. C, Ratio distribution of the proteins identified in the TE, from secretory organelles. MIC, micronemes (yellow); ROP, rhoptries (green); GRA, dense granules (blue); surface (light pink).