Schematic representation of identified proteins between both approaches: secreted fraction (ESA) and relative dimethyl quantification (QUANT). N. caninum proteins identified in the present study were grouped according to their known or putative localisation. Proteins in the purple zone were identified by the ESA approach; proteins in the green zone were identified by the quantitative approach; proteins in the intersection of purple and green zones were identified by both approaches. ESA proteins are arranged in descending order of protein abundance. QUANT proteins in red belong to the down-regulated group, and in yellow were classified as not differentially expressed.